Fig 1: Effect of HIFPL on MCP-1 and IL-8 levels in carrageenan-induced paw edema in mice. HIFPL was tested at 40, 80 and 160 mg/kg (oral administration) and indomethacin (5 mg/kg, oral administration) was used as a reference drug. The values are mean ± standard errors. * p < 0.05; n.s.: not significant (ANOVA/Dunnett), compared to the solvent-treated group.
Fig 2: LECT2 reduced mRNA expression and serum concentrations of inflammatory cytokines and chemokines in Apoe – / – mice mRNA expression of IL-1β (A), IL-8(B), TNF-α(C), MCP-1(D), and MMP-1(E) was quantified by qRT-PCR (n=5 per group). And the serum concentrations were determined using enzyme-linked immunosorbent assay (n=8 per group). Data are means±SD. #: P<0.05, ##: P<0.01, vs. control; *: P<0.05, **: P<0.01, vs. AS group.
Fig 3: CXCL10 promotes secretions of inflammatory mediators of macrophage via CXCR3-mediated ERK and p38 MAPK activation. The mRNA expression levels of IL-6 (A) and MCP1 (B) in BMDMs were calculated 3 h after LPS treatment. BMDMs from EAP mice were pretreated with AMG487 (1 μM) for 1 h, then stimulated with CXCL10 (100 ng/ml) for 12 h, and then with LPS (50 ng/ml) for 3 h. Cells were collected for detecting the mRNA expression levels of IL-6 (A) and MCP1 (B). (C) Western blot analysis of the phosphorylation of the ERK1/2, and p38 MAPK signaling pathways in LPS-induced macrophages for 3 h. The expression levels of IL-6 in the prostate (D) and serum (E) for mice in the normal, EAP, sh-NC, and sh-CXCL10 groups. The expression levels of IL-6 in the prostate (F) and serum (G) for mice in the AMG487 and vehicle groups. The expression levels of MCP1 in the prostate (H) and serum (I) for mice in the normal, EAP, sh-NC, and sh-CXCL10 groups. The expression levels of MCP1 in the prostate (J) and serum (K) for mice in the AMG487 and vehicle groups. Data are shown as mean ± SD by one-way ANOVA analysis (A, B) or unpaired, two-tailed Student’s t-test analysis (D–K). *P < 0.05; **P < 0.01. ns, no significance.
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